NADH/NAD+ disruption

What it is

Ethanol metabolism via ADH + ALDH produces 2 NADH per ethanol molecule. Normal cellular NADH/NAD+ ratio is ~0.01; ethanol dramatically shifts this toward NADH.

Downstream consequences

Gluconeogenesis inhibition

  • NADH elevation inhibits the malate-aspartate shuttle and LDH reverse reaction
  • Blocks conversion of lactate → pyruvate → glucose
  • Net effect: hypoglycaemia (particularly on empty stomach) + lactate accumulation
  • This contributes to fatigue, brain fog, dizziness during hangover

Fatty acid metabolism disruption

  • NADH inhibits β-hydroxyacyl-CoA dehydrogenase
  • β-oxidation slowed → fatty acids accumulate
  • Accumulated NADH + acetate drive fatty acid synthesis
  • Early stage: hepatic steatosis (fatty liver)

Redox feedback loop

  • Peroxisomal β-oxidation also generates NADH
  • Creates a feedback inhibition loop limiting ethanol oxidation rate
  • Catalase pathway (uses H₂O₂) does NOT produce NADH — may help mitigate redox stress

Hangover contribution

Hypoglycaemia from NADH/NAD+ disruption is a direct contributor to next-day:

  • Fatigue and weakness
  • Brain fog and impaired executive function
  • Difficulty concentrating

Recovery

NADH/NAD+ ratio normalises as ethanol clears — timeline depends on dose and metabolic capacity.

Alcohol, CYP2E1 and ROS, Hangover mechanism